This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Antigen retrieval with Target Retrieval Solution, pH9 (Agilent Technologies, Cat. #S2367) for 40 minutes at 95°C.
Primary and secondary incubations performed using the following reagents:
Antibody | Source | Catalog # | Lot # | RRID # | Working Dilution/Concentration |
Anti-ZO-1 | Invitrogen | 61-7300 | 1452279A | AB_2533938 | 5 µg/ml |
Anti-E Cadherin antibody [M168] | Abcam | ab76055 | GR216035 | AB_1310159 | 0.83 µg/ml |
Anti-Actin antibody [MAC 237] | Abcam | ab50591 | GR168034 | AB_867488 | 8.2 µg/ml |
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Alexa Fluor® 488 AffiniPure Donkey Anti-Mouse IgG (H+L) | Jackson Immunoresearch | 715-545-151 | 118237 | AB_2341099 | 1.5 µg/ml |
Alexa Fluor® 594 AffiniPure F(ab’)₂ Fragment Donkey Anti-Rabbit IgG (H+L) | Jackson Immunoresearch | 711-586-152 | 124065 | AB_2340622 | 1.5 µg/ml |
Alexa Fluor® 647 AffiniPure F(ab’)₂ Fragment Donkey Anti-Rat IgG (H+L) | Jackson Immunoresearch | 712-606-153 | 123491 | AB_2340696 | 1.5 µg/ml |
Hoechst 33342 | Life Technologies | H3570 |
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| 10 µg/ml |
Brigham and
Women's
Hospital
Harvard
Medical
School
© Copyright JR Turner 2020.