This gene encodes an intestinal hydrogen peptide cotransporter that is a member of the solute carrier family 15. The encoded protein is localized to the brush border membrane of the intestinal epithelium and mediates the uptake of di- and tripeptides from the lumen into the enterocytes. This protein plays an important role in the uptake and digestion of dietary proteins. This protein also facilitates the absorption of numerous peptidomimetic drugs. [provided by RefSeq, Apr 2010]

Antibody charactierzation and validation (SLC15A1):

This antibody was produced by immunizing rabbits with a synthetic peptide that corresponds to the C-terminal 20 amino acids of human SLC15A1/PEPT1 protein (sequence NRLEKSNPYFMSGANSQKQN) coupled with key-hole limpet haemocyanin (Merlin et al., 2001*). The antibodies are affinity purified against the synthetic peptide. In formalin-fixed, paraffin-embedded tissues, the antibody recognizes human, but not mouse, PEPT1. It is expressed in the brush border of small intestinal epithelium (right, upper image), but not in the colon (right, lower image).