The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] 

Antibody charactierzation and validation (SLC12A2):

This commercial rabbit polycolonal antibody from Abcam* (ab59791) was raised against “synthetic peptide derived from the N terminal region of human NKCC1.” Antibodies are purified from whole serum. In formalin-fixed, paraffin-embedded tissue, the antibody recognizes both human and mouse. It shows basolateral localization predominantly in the crypts for both small intestine (right, upper image) and colon. The 3x zoomed in image (right, lower image) confirms the basolateral localization.