This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Immunostaining procedure conditions:
Antigen retrieval with Target Retrieval Solution, pH6 (Agilent Technologies, Cat. #S1699) for 15 minutes at 120°C.
Primary and secondary incubations performed using the following reagents:
Antibody | Source | Catalog # | Lot # | RRID # | Working Dilution/Concentration |
Anti-SGLT1 antibody | Abcam | Ab14686 | GR306587-11 | AB_301411 | 8.5 µg/ml |
Anti-E Cadherin antibody [M168] | Abcam | ab76055 | GR26008-7 | AB_1310159 | 1:300 |
Anti-ZO-1 [1F2G12] |
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| AB_2783875 | 0.16 µg/ml |
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Alexa Fluor® 488 AffiniPure Donkey Anti-Mouse IgG (H+L) | Jackson Immunoresearch | 715-545-151 | 118237 | AB_2341099 | 1.5 µg/ml |
Alexa Fluor® 594 AffiniPure F(ab’)₂ Fragment Donkey Anti-Rabbit IgG (H+L) | Jackson Immunoresearch | 711-586-152 | 114063 | AB_2340622 | 1.5 µg/ml |
Alexa Fluor® 647 AffiniPure F(ab’)₂ Fragment Donkey Anti-Rat IgG (H+L) | Jackson Immunoresearch | 712-606-153 | 123491 | AB_2340696 | 1.5 µg/ml |
Hoechst 33342 | Life Technologies | H3570 |
|
| 10 µg/ml |
Brigham and
Women's
Hospital
Harvard
Medical
School
© Copyright JR Turner 2020.