This gene belongs to the solute carrier 26 family, whose members encode anion transporter proteins. This particular family member encodes a protein involved in transporting chloride, oxalate, sulfate and bicarbonate. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Aug 2013]
Immunostaining procedure conditions:
Antigen retrieval with Target Retrieval Solution, pH6 (Agilent Technologies, Cat. #S1699) for 15 minutes at 120°C.
Primary and secondary incubations performed using the following reagents:
Antibody | Source | Catalog # | Lot # | RRID # | Working Dilution/Concentration |
Anti-SLC26A6 antibody | Aronson Lab |
|
|
| 1:1000 |
Anti-E Cadherin antibody [M168] | Abcam | ab76055 | GR216035-5 | AB_1310159 | 1:300 |
ZO-1 [R40.76] |
|
|
| AB_27838859 | 0.16 µg/ml |
|
|
|
|
|
|
|
|
|
|
|
|
Alexa Fluor® 488 AffiniPure F(ab’)2 Donkey Anti-Mouse IgG (H+L) | Jackson Immunoresearch | 715-546-151 | 138115 | AB_2340850 | 1.5 µg/ml |
Alexa Fluor® 594 AffiniPure F(ab’)₂ Fragment Donkey Anti-Rabbit IgG (H+L) | Jackson Immunoresearch | 711-586-152 | 136184 | AB_2340622 | 1.5 µg/ml |
Alexa Fluor® 647 AffiniPure F(ab’)₂ Fragment Donkey Anti-Rat IgG (H+L) | Jackson Immunoresearch | 712-606-153 | 123491 | AB_2340696 | 1.5 µg/ml |
Hoechst 33342 | Life Technologies | H3570 |
|
| 1.5 µg/ml |
*Generously provided by Peter S. Aronson, M.D., Yale University
Brigham and
Women's
Hospital
Harvard
Medical
School
© Copyright JR Turner 2020.