This gene encodes a member of the solute carrier/sulfate transporter family. The encoded protein is predominantly expressed in the intestine where it is essential for chloride absorption. Disruption of this gene results in chloride-rich diarrhea and compensatory up-regulation of ion-absorbing transporters. [provided by RefSeq, Dec 2012]
Immunostaining procedure conditions:
Antigen retrieval with Target Retrieval Solution, pH6 (Agilent Technologies, Cat. #S1699) for 15 minutes at 120°C.
Primary and secondary incubations performed using the following reagents:
Antibody | Source | Catalog # | Lot # | RRID # | Working Dilution/Concentration |
DRA | Santa Cruz | sc-376187 | B116265 | AB_10990725 | 1:100 |
Anti-E Cadherin antibody [M168] | Abcam | ab76055 | GR26008-7 | AB_1310159 | 1:300 |
ZO-1 [R40.76] |
|
|
| AB_2783859 | 0.16 µg/ml |
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|
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|
|
|
|
|
|
|
Alexa Fluor® 488 AffiniPure F(ab’)2 Donkey Anti-Mouse IgG (H+L) | Jackson Immunoresearch | 715-546-151 | 138115 | AB_2340850 | 1.5 µg/ml |
Alexa Fluor® 594 AffiniPure F(ab’)₂ Fragment Donkey Anti-Rabbit IgG (H+L) | Jackson Immunoresearch | 711-586-152 | 136184 | AB_2340622 | 1.5 µg/ml |
Alexa Fluor® 647 AffiniPure F(ab’)₂ Fragment Donkey Anti-Rat IgG (H+L) | Jackson Immunoresearch | 712-606-153 | 123491 | AB_2340696 | 1.5 µg/ml |
Hoechst 33342 | Life Technologies | H3570 |
|
| 1.5 µg/ml |
Brigham and
Women's
Hospital
Harvard
Medical
School
© Copyright JR Turner 2020.