Le Shen : Turner Lab

Le Shen, M.D., Ph.D.

Ph.D. student (2002-2005), Postdoctoral Fellow (2005-2011), Assistant Professor (2011-2016)

2002-2016

Current Position:
Assistant Professor, The University of Chicago

Crohn’s and Colitis Foundation of America Reseach Fellow
K08 recipient

Publications

Kuo WT, Shen L, Zuo L, Shashikanth N, Ong M, Wu L, Zha J, Edelblum KL, Wang Y, Wang Y, Nilsen SP, Turner JR. Inflammation-induced occludin downregulation limits epithelial apoptosis by suppressing caspase-3 expression. 2019. Gastroenterology. 157, 1323-1337. PMC6815722
SUMMARY PDF PUBMED
Odenwald MA, Choi W, Kuo WT, Singh G, Sailer A, Wang Y, Shen L, Fanning AS, Turner JR. The scaffolding protein ZO-1 coordinates actomyosin and epithelial apical specializations in vitro and in vivo. 2018. J Biol Chem. 293, 17317-17335. PMC6231134
SUMMARY PDF PUBMED
Tsai PY, Zhang B, He WQ, Zha JM, Odenwald MA, Singh G, Tamura A, Shen L, Sailer A, Yeruva S, Kuo WT, Fu YX, Tsukita S, Turner JR. IL-22 upregulates epithelial claudin-2 to drive diarrhea and enteric pathogen clearance. 2017. Cell Host Microbe. 21, 671-681 e674. PMC5541253
SUMMARY PDF PUBMED
Mir H, Meena AS, Chaudhry KK, Shukla PK, Gangwar R, Manda B, Padala MK, Shen L, Turner JR, Dietrich P, Dragatsis I, Rao R. Occludin deficiency promotes ethanol-induced disruption of colonic epithelial junctions, gut barrier dysfunction and liver damage in mice. 2016. Biochim Biophys Acta. 1860, 765-774. PMC4776745
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Weber CR, Liang GH, Wang Y, Das S, Shen L, Yu AS, Nelson DJ, Turner JR. Claudin-2-dependent paracellular channels are dynamically gated. 2015. Elife. 4, e09906. PMC4755754
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Pan J, Zhang L, Odenwald MA, Shen L, Turner JR, Bergelson JM. Expression of human decay-accelerating factor on intestinal epithelium of transgenic mice does not facilitate infection by the enteral route. 2015. J Virol. 89, 4311-4318. PMC4442383
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Turner JR, Buschmann MM, Romero-Calvo I, Sailer A, Shen L. The role of molecular remodeling in differential regulation of tight junction permeability. 2014. Semin Cell Dev Biol. 36, 204-212. PMC4253049
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Ma K, Malhotra P, Soni V, Hedroug O, Annaba F, Dudeja A, Shen L, Turner JR, Khramtsova EA, Saksena S, Dudeja PK, Gill RK, Alrefai WA. Overactivation of intestinal SREBP2 in mice increases serum cholesterol. 2014. PLoS ONE. 9, e84221. PMC3896331
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Bouyer PG, Tang X, Weber CR, Shen L, Turner JR, Matthews JB. Capsaicin induces NKCC1 internalization and inhibits chloride secretion in colonic epithelial cells independently of TRPV1. 2013. Am J Physiol Gastrointest Liver Physiol. 304, G142-156. PMC3543646
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Su L, Nalle SC, Shen L, Turner ES, Singh G, Breskin LA, Khramtsova EA, Khramtsova G, Tsai PY, Fu YX, Abraham C, Turner JR. TNFR2 activates MLCK-dependent tight junction dysregulation to cause apoptosis-mediated barrier loss and experimental colitis. 2013. Gastroenterology. 145, 407-415. PMC3722284
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Buschmann MM, Shen L, Rajapakse H, Raleigh DR, Wang Y, Wang Y, Lingaraju A, Zha J, Abbott E, McAuley EM, Breskin LA, Wu L, Anderson K, Turner JR, Weber CR. Occludin OCEL-domain interactions are required for maintenance and regulation of the tight junction barrier to macromolecular flux. 2013. Mol Biol Cell. 24, 3056-3068. PMC3784380
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Edelblum KL, Shen L, Weber CR, Marchiando AM, Clay BS, Wang Y, Prinz I, Malissen B, Sperling AI, Turner JR. Dynamic migration of gammadelta intraepithelial lymphocytes requires occludin. 2012. Proc Natl Acad Sci U S A. 109, 7097-7102. PMC3345021
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Guan Y, Watson AJ, Marchiando AM, Bradford E, Shen L, Turner JR, Montrose MH. Redistribution of the tight junction protein ZO-1 during physiological shedding of mouse intestinal epithelial cells. 2011. Am J Physiol Cell Physiol. 300, C1404-1414. PMC3118625
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Raleigh DR, Boe DM, Yu D, Weber CR, Marchiando AM, Bradford EM, Wang Y, Wu L, Schneeberger EE, Shen L, Turner JR. Occludin S408 phosphorylation regulates tight junction protein interactions and barrier function. 2011. J Cell Biol. 193, 565-582. PMC3087007
SUMMARY PDF PUBMED
Marchiando AM, Shen L, Graham WV, Edelblum KL, Duckworth CA, Guan Y, Montrose MH, Turner JR, Watson AJ. The epithelial barrier is maintained by in vivo tight junction expansion during pathologic intestinal epithelial shedding. 2011. Gastroenterology. 140, 1208-1218 e1201-1202. PMC3066304
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Shen L, Weber CR, Raleigh DR, Yu D, Turner JR. Tight junction pore and leak pathways: a dynamic duo. 2011. Annual review of physiology. 73, 283-309. PMC4655434
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Weber CR, Raleigh DR, Su L, Shen L, Sullivan EA, Wang Y, Turner JR. Epithelial myosin light chain kinase activation induces mucosal interleukin-13 expression to alter tight junction ion selectivity. 2010. J Biol Chem. 285, 12037-12046. PMC2852941
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Mykoniatis A, Shen L, Fedor-Chaiken M, Tang J, Tang X, Worrell RT, Delpire E, Turner JR, Matlin KS, Bouyer P, Matthews JB. Phorbol 12-myristate 13-acetate-induced endocytosis of the Na-K-2Cl cotransporter in MDCK cells is associated with a clathrin-dependent pathway. 2010. Am J Physiol Cell Physiol. 298, C85-97. PMC2806153
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Marchiando AM, Shen L, Graham WV, Weber CR, Schwarz BT, Austin JR, 2nd, Raleigh DR, Guan Y, Watson AJ, Montrose MH, Turner JR. Caveolin-1-dependent occludin endocytosis is required for TNF-induced tight junction regulation in vivo. 2010. J Cell Biol. 189, 111-126. PMC2854371
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Raleigh DR, Marchiando AM, Zhang Y, Shen L, Sasaki H, Wang Y, Long M, Turner JR. Tight junction-associated MARVEL proteins marveld3, tricellulin, and occludin have distinct but overlapping functions. 2010. Mol Biol Cell. 21, 1200-1213. PMC2847524
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Yu D, Marchiando AM, Weber CR, Raleigh DR, Shen L, Turner JR, Wang Y. MLCK-dependent exchange and actin binding region-dependent anchoring of ZO-1 regulate tight junction barrier function. 2010. Proc Natl Acad Sci U S A. 107, 8237-8241. PMC2889526
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Elias BC, Suzuki T, Seth A, Giorgianni F, Kale G, Shen L, Turner JR, Naren A, Desiderio DM, Rao R. Phosphorylation of Tyr-398 and Tyr-402 in occludin prevents its interaction with ZO-1 and destabilizes its assembly at the tight junctions. 2009. J Biol Chem. 284, 1559-1569. PMC2615497
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Graham WV, Marchiando AM, Shen L, Turner JR. No static at all. 2009. Ann N Y Acad Sci. 1165, 314-322. PMC2768559
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Suzuki T, Elias BC, Seth A, Shen L, Turner JR, Giorgianni F, Desiderio D, Guntaka R, Rao R. PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. 2009. Proc Natl Acad Sci U S A. 106, 61-66. PMC2629239
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Su L, Shen L, Clayburgh DR, Nalle SC, Sullivan EA, Meddings JB, Abraham C, Turner JR. Targeted epithelial tight junction dysfunction causes immune activation and contributes to development of experimental colitis. 2009. Gastroenterology. 136, 551-563. PMC2712351
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Shen L, Su L, Turner JR. Mechanisms and functional implications of intestinal barrier defects. 2009. Dig Dis. 27, 443-449. PMC2814011
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Liu S, Yang W, Shen L, Turner JR, Coyne CB, Wang T. Tight junction proteins claudin-1 and occludin control hepatitis C virus entry and are downregulated during infection to prevent superinfection. 2009. J Virol. 83, 2011-2014. PMC2643775
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Mukherjee A, Morosky SA, Shen L, Weber CR, Turner JR, Kim KS, Wang T, Coyne CB. Retinoic acid-induced gene-1 (RIG-I) associates with the actin cytoskeleton via caspase activation and recruitment domain-dependent interactions. 2009. J Biol Chem. 284, 6486-6494. PMC5405323
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Wroblewski LE, Shen L, Ogden S, Romero-Gallo J, Lapierre LA, Israel DA, Turner JR, Peek RM, Jr. Helicobacter pylori dysregulation of gastric epithelial tight junctions by urease-mediated myosin II activation. 2009. Gastroenterology. 136, 236-246. PMC2678540
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Shen L, Turner JR. Intercellular junctions: actin the PARt. 2008. Curr Biol. 18, R1014-1017
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Shen L, Weber CR, Turner JR. The tight junction protein complex undergoes rapid and continuous molecular remodeling at steady state. 2008. J Cell Biol. 181, 683-695. PMC2386107
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Gill RK, Shen L, Turner JR, Saksena S, Alrefai WA, Pant N, Esmaili A, Dwivedi A, Ramaswamy K, Dudeja PK. Serotonin modifies cytoskeleton and brush-border membrane architecture in human intestinal epithelial cellsg. 2008. Am J Physiol Gastrointest Liver Physiol. 295, G700-708. PMC2575920
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Schwarz BT, Wang F, Shen L, Clayburgh DR, Su L, Wang Y, Fu YX, Turner JR. LIGHT signals directly to intestinal epithelia to cause barrier dysfunction via cytoskeletal and endocytic mechanisms. 2007. Gastroenterology. 132, 2383-2394. PMC2709832
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Coyne CB, Shen L, Turner JR, Bergelson JM. Coxsackievirus entry across epithelial tight junctions requires occludin and the small GTPases Rab34 and Rab5. 2007. Cell Host Microbe. 2, 181-192. PMC2719558
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Shen L, Turner JR. Role of epithelial cells in initiation and propagation of intestinal inflammation. Eliminating the static: tight junction dynamics exposed. 2006. Am J Physiol Gastrointest Liver Physiol. 290, G577-582
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Shen L, Black ED, Witkowski ED, Lencer WI, Guerriero V, Schneeberger EE, Turner JR. Myosin light chain phosphorylation regulates barrier function by remodeling tight junction structure. 2006. J Cell Sci. 119, 2095-2106
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Wu L, Estrada O, Zaborina O, Bains M, Shen L, Kohler JE, Patel N, Musch MW, Chang EB, Fu YX, Jacobs MA, Nishimura MI, Hancock RE, Turner JR, Alverdy JC. Recognition of host immune activation by Pseudomonas aeruginosa. 2005. Science. 309, 774-777
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Shen L, Turner JR. Actin depolymerization disrupts tight junctions via caveolae-mediated endocytosis. 2005. Mol Biol Cell. 16, 3919-3936. PMC1196308
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Russo JM, Florian P, Shen L, Graham WV, Tretiakova MS, Gitter AH, Mrsny RJ, Turner JR. Distinct temporal-spatial roles for rho kinase and myosin light chain kinase in epithelial purse-string wound closure. 2005. Gastroenterology. 128, 987-1001. PMC1237051
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Clayburgh DR, Shen L, Turner JR. A porous defense: the leaky epithelial barrier in intestinal disease. 2004. Lab Invest. 84, 282-291
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Brigham and
Women's
Hospital

Harvard
Medical
School

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