Claudin-4 old

Claudin-4 is a structural component of the tight junction that enhances the paracellular barrier.
Antibody validation This commercial rabbit antibody from Abcam (ab210796) is a monoclonal antibody raised against a synthetic peptide derived from the C-terminal region of the human claudin-4. Our validation included staining of formalin-fixed, paraffin-embedded human duodenum, where the antibody decorated tight junctions throughout the crypt-villus axis.

Immunostaining procedure conditions
Antigen retrieval with Target Retrieval Solution, pH9 (Agilent Technologies, Cat. #S2367) for 40 minutes at 95°C.
Primary and secondary incubations performed using the following reagents:

Antibody

Source

Catalog #

Lot #

RRID #

Working Dilution/Concentration
Anti-claudin-4 antibody Abcam ab210796 GR264117-1 AB_2732879 10 µg/ml
Anti-E-cadherin antibody [M168] Abcam ab76055 GR216035-5 AB_1310159 2.5 µg/ml
Anti-actin antibody Abcam ab50591 GR264117-1 AB_867488 5.65 µg/ml
Alexa Fluor 488 AffiniPure F(ab’)2 donkey anti-mouse IgG (H+L) Jackson Immunoresearch 715-545-151 130065 AB_2341099 1.5 µg/ml
Alexa Fluor 594 AffiniPure F(ab’)₂ Donkey anti-rabbit IgG (H+L) Jackson Immunoresearch 711-586-152 124065 AB_2340622 1.5 µg/ml
Alexa Fluor 647 AffiniPure F(ab’)₂ donkey anti-rat IgG (H+L) Jackson Immunoresearch 712-606-153 123491 AB_2340696 1.5 µg/ml
Hoechst 33342 Life Technologies H3570 10 µg/ml
Duodenum

Jerrold R. Turner, MD, PhD
Professor of Pathology and Medicine

Brigham and
Women's
Hospital

Harvard
Medical
School

TH 613
20 Shattuck St.
Boston, MA 02115.

© Copyright JR Turner 2020.