SLC6A19 is a transmembrane protein that actively transports most neutral amino acids across the apical membrane. Mutations result in Hartnup disorder. 

Antibody validation
This commercial rabbit antibody from Atlas Antibodies (HPA043207) is a polyclonal affinity isolated antibody raised agasint the sequence MVRLVLPNPGLDARIPSLAELETIEQEEASSRPKWDNK. Enhanced validation by the manufacturer was by comparison of IHC stains to RNASeq data. Our validation included staining of formalin-fixed, paraffin-embedded human duodenum, where SLC6A19 is detected within the apical brush border of villous, but not crypt, epithelial cells (right, upper image). In contrast, expression is not detected in the colon (right, lower image), as expected.

Immunostaining 
Antigen retrieval with Target Retrieval Solution, pH9 (Agilent Technologies, Cat. #S2367) for 40 minutes at 95°C.
Primary and secondary incubations performed used the following reagents:

Antibody

Source

Catalog #

Lot #

RRID #

Working Dilution/Concentration

Rabbit anti-B0AT1 antibody Atlas Antibodies

HPA043207

R41029

AB_2678363

3 µg/ml

Mouse anti-E Cadherin antibody [M168] Abcam

ab76055

GR21603-5

AB_1310159

1:300

Rat anti-ZO-1 [1F2G12]  Turner lab

AB_2783875

1:50

   

   

Alexa Fluor® 488 AffiniPure F(ab’)2 Donkey Anti-Mouse IgG (H+L) Jackson Immunoresearch

715-546-151

130065

AB_2341099

3 µg/ml

Alexa Fluor® 594 AffiniPure F(ab’)₂ Fragment Donkey Anti-Rabbit IgG (H+L) Jackson Immunoresearch

711-586-152

114063

AB_2340622

3 µg/ml

Alexa Fluor® 647 AffiniPure F(ab’)₂ Fragment Donkey Anti-Rat IgG (H+L) Jackson Immunoresearch

712-606-153

123491

AB_2340696

3 µg/ml

Hoechst 33342 Life Technologies

H3570

10 µg/ml

Duodenum

Colon